Human G-CSF/CSF3 ELISA Kit

96T/2480

C17orf33; CSF3; Filgrastim; G CSF; GCSF; G-CSF; Lenograstim; Pluripoietin

E.coli, T31-P204

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2000 pg/ml

CSF3

colony stimulating factor 3 (granulocyte)

P09919  

Human G-CSF/CSF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0360)

Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

25-30分钟

Catalog number	Description	Quantity
EK0360-CAP	96-well plate precoated with anti- human G-CSF antibody	1
EK0360-ST	Lyophilized recombinant human G-CSF standard	10ng/tube×2
EK0360-DA	Biotinylated anti- human G-CSF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	62	237	1001	64	230	966
Standard deviation	3.96	15.16	45.04	4.99	18.17	59.89
CV(%)	6.4	6.4	4.5	7.8	7.9	6.2

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.011	0.135	0.279	0.464	0.928	1.496	1.956	2.308

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h