Human G-CSF/CSF3 Quick ELISA Kit

96T/3580

C17orf33; CSF3; Filgrastim; G CSF; GCSF; G-CSF; Lenograstim; Pluripoietin

Expression system for standard: E.coli; Immunogen sequence: T31-P204

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

CSF3

colony stimulating factor 3 (granulocyte)

P09919  

Human G-CSF/CSF3 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0360)

Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

15分钟

Catalog number	Description	Quantity
FEK0360-CAP	Anti-human G-CSF Pre-coated 96-well Strip Microplate	1
FEK0360-ST	human G-CSF standard	10ng/tube×2
FEK0360-H	HRP Linked Anti-human G-CSF Antibody	6ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	62	237	1001	64	230	966
Standard deviation	3.96	15.16	45.04	4.99	18.17	59.89
CV(%)	6.4%	6.4%	4.5%	7.8%	7.9%	6.2%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.058	0.092	0.170	0.319	0.599	1.080	1.902

Store at 4℃ for 6 months.

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)