产品名称
Human GDNF ELISA Kit
指标别名
GDNF; HFB1 GDNF; hGDNF; ATF; ATF1; ATF2; HFB1-GDNF; HSCR3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
glial cell derived neurotrophic factor
文献引用格式
Human GDNF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0362)
背景资料
Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0362-CAP | 96-well plate precoated with anti- human GDNF antibody | 1 |
EK0362-ST | Lyophilized recombinant human GDNF standard | 10ng/tube×2 |
EK0362-DA | Biotinylated anti- human GDNF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 69 | 264 | 855 | 66 | 242 | 810 |
Standard deviation | 4.96 | 17.16 | 36.76 | 6 | 16.45 | 37.26 |
CV(%) | 7.2 | 6.5 | 4.3 | 9.1 | 6.8 | 4.6 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.006 | 0.103 | 0.185 | 0.353 | 0.685 | 1.066 | 1.644 | 2.246 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)