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Rat GDNF ELISA Kit

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EK0363

  • 96T ¥2280
  • 货期: 现货

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产品名称
Rat GDNF ELISA Kit
规格/价格
96T/2280
指标别名
GDNF; HFB1 GDNF; hGDNF; ATF; ATF1; ATF2; HFB1-GDNF; HSCR3
免疫原
Expression system for standard: NS0; Immunogen sequence: S78-I211
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
GDNF
基因名全称
glial cell derived neurotrophic factor
Uniprot ID
Q07731  
文献引用格式
Rat GDNF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0363)
背景资料
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.
试剂盒内容
Catalog numberDescriptionQuantity
EK0363-CAP96-well plate precoated with anti- rat GDNF antibody1
EK0363-STLyophilized recombinant rat GDNF standard10ng/tube×2
EK0363-DABiotinylated anti- rat GDNF antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)90280983942641071
Standard deviation4.3222.1269.795.5424.5584.6
CV(%)4.8%7.9%7.1%5.9%9.3%7.9%
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0100.0690.0990.1950.3890.7901.4692.305
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

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