产品名称
Human Gp130/IL6ST ELISA Kit
指标别名
CD130; CDw130; Glycoprotein 130; GP130; GP130 RAPS; IL 6 receptor subunit beta; IL 6R beta; IL 6R subunit beta; IL 6RB; IL6R beta; IL-6RB; IL6ST; IL-6ST; IL 6ST; Membrane glycoprotein 130
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
125 pg/ml - 8000 pg/ml
基因名全称
interleukin 6 signal transducer
文献引用格式
Human Gp130/IL6ST ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0367)
背景资料
GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane proteinwhich is the founding member of the class of all℃ytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-boundgp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0367-CAP | 96-well plate precoated with anti- human GP130 antibody | 1 |
EK0367-ST | Lyophilized recombinant human GP130 standard | 10ng/tube×2 |
EK0367-DA | Biotinylated anti- human GP130 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 186 | 1115 | 3384 | 178 | 1076 | 3150 |
Standard deviation | 13.2 | 73.59 | 155.66 | 12.63 | 80.7 | 198.45 |
CV(%) | 7.1 | 6.6 | 4.6 | 7.1 | 7.5 | 6.3 |
典型数据示例
Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
O.D. | 0.035 | 0.109 | 0.179 | 0.286 | 0.543 | 0.970 | 1.466 | 2.203 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)