产品名称
Rat Gp130/IL6ST ELISA Kit
指标别名
CD130; CDw130; Glycoprotein 130; GP130; GP130 RAPS; IL 6 receptor subunit beta; IL 6R beta; IL 6R subunit beta; IL 6RB; IL6R beta; IL-6RB; IL6ST; IL-6ST; IL 6ST; Membrane glycoprotein 130
免疫原
Expression system for standard: NS0; Immunogen sequence: Q23-E618
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
interleukin 6 signal transducer
文献引用格式
Rat Gp130/IL6ST ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1742)
背景资料
GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
试剂盒内容
Catalog number | Description | Quantity |
EK1742-CAP | 96-well plate precoated with anti- rat IL6ST antibody | 1 |
EK1742-ST | Lyophilized recombinant rat IL6ST standard | 50ng/tube×2 |
EK1742-DA | Biotinylated anti- rat IL6ST antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1254 | 4579 | 20188 | 1322 | 5546 | 21456 |
Standard deviation | 66.46 | 290.3 | 847.9 | 99.15 | 321.7 | 1652.1 |
CV(%) | 5.3% | 6.1% | 4.2% | 7.5% | 5.8% | 7.7% |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.008 | 0.329 | 0.516 | 0.817 | 1.102 | 1.336 | 1.568 | 1.839 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)