指标别名
insulin like growth factor 2; C11orf43; GRDF; IGF-II; PP9974; SRS3; IGF-2; IGF2
免疫原
Expression system for standard: E.coli; Immunogen sequence: A25-E91
交叉反应
There is no detectable cross-reactivity with IGF-1.
基因名全称
insulin-like growth factor 2 (somatomedin A)
文献引用格式
Rat IGF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0380)
背景资料
Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
试剂盒内容
Catalog number | Description | Quantity |
EK0380-CAP | 96-well plate precoated with anti- rat IGF-2 antibody | 1 |
EK0380-ST | Lyophilized recombinant rat IGF-2 standard | 10ng/tubex2 |
EK0380-DA | Biotinylated anti- rat IGF-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 144 | 684 | 1763 | 153 | 684 | 1731 |
Standard deviation | 5.76 | 33.51 | 84.62 | 7.65 | 45.82 | 114.24 |
CV(%) | 4% | 4.9% | 4.8% | 5% | 6.7% | 6.6% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.049 | 0.112 | 0.196 | 0.309 | 0.593 | 1.040 | 1.668 | 2.171 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)