产品名称
Chinese Hamster IGF2 ELISA Kit
指标别名
insulin like growth factor 2; C11orf43; GRDF; IGF-II; PP9974; SRS3; IGF-2; IGF2
免疫原
Expression system for standard: E.coli; Immunogen sequence: A25-E91
交叉反应
There is no detectable cross-reactivity with IGF-1.
基因名全称
Insulin-like growth factor II
文献引用格式
Chinese Hamster IGF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0380-HA)
背景资料
Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
试剂盒内容
Catalog number | Description | Quantity |
EK0380-HA-CAP | Anti-Hamster Igf2 Pre-coated 96-well strip microplate | 1 |
EK0380-HA-ST | Hamster Igf2 Standard | 10ng/tube×2 |
EK0380-HA-DA | Hamster Igf2 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 478 | 1536 | 2743 | 453 | 1737 | 2968 |
Standard deviation | 20.55 | 87.55 | 126.2 | 30.8 | 140.7 | 222.6 |
CV (%) | 4.3 | 5.7 | 4.6 | 6.8 | 8.1 | 7.5 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
O.D. | 0.049 | 0.112 | 0.196 | 0.309 | 0.593 | 1.04 | 1.668 | 2.171 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)