产品名称
Human IGFBP1 ELISA Kit
指标别名
AFBP; hIGFBP 1; IBP 1; IBP1; IGF binding protein 1; IGF BP25; IGFBP 1; IGFBP1; Placental protein 12; PP12
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
insulin-like growth factor binding protein 1
文献引用格式
Human IGFBP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0382)
背景资料
IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0382-CAP | 96-well plate precoated with anti- human IGFBP-1 antibody | 1 |
EK0382-ST | Lyophilized recombinant human IGFBP-1 standard | 10ng/tubex2 |
EK0382-DA | Biotinylated anti- human IGFBP-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 66 | 243 | 1016 | 68 | 225 | 1048 |
Standard deviation | 3.23 | 17.25 | 67.05 | 3.67 | 19.8 | 86.98 |
CV(%) | 4.9 | 7.1 | 6.6 | 5.4 | 8.8 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.052 | 0.140 | 0.225 | 0.386 | 0.701 | 1.134 | 1.941 | 2.626 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine