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Human IGFBP1 ELISA Kit

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EK0382

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human IGFBP1 ELISA Kit
规格/价格
96T/2480
指标别名
AFBP; hIGFBP 1; IBP 1; IBP1; IGF binding protein 1; IGF BP25; IGFBP 1; IGFBP1; Placental protein 12; PP12
免疫原
NSO, A26-N259
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
IGFBP1
基因名全称
insulin-like growth factor binding protein 1
Uniprot ID
P08833  
文献引用格式
Human IGFBP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0382)
背景资料
IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0382-CAP96-well plate precoated with anti- human IGFBP-1 antibody1
EK0382-STLyophilized recombinant human IGFBP-1 standard10ng/tubex2
EK0382-DABiotinylated anti- human IGFBP-1 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)662431016682251048
Standard deviation3.2317.2567.053.6719.886.98
CV(%)4.97.16.65.48.88.3
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0520.1400.225 0.3860.7011.1341.9412.626
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
实验时长
3.5h

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