产品名称
Mouse IGFBP1 ELISA Kit
指标别名
AFBP; hIGFBP 1; IBP 1; IBP1; IGF binding protein 1; IGF BP25; IGFBP 1; IGFBP1; Placental protein 12; PP12
免疫原
Expression system for standard: NS0; Immunogen sequence: A26-N272
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Insulin-like growth factor-binding protein 1
文献引用格式
Mouse IGFBP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0383)
背景资料
IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0383-CAP | 96-well plate precoated with anti- mouse IGFBP-1 antibody | 1 |
EK0383-ST | Lyophilized recombinant mouse IGFBP-1 standard | 10ng/tubex2 |
EK0383-DA | Biotinylated anti- mouse IGFBP-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 88 | 281 | 865 | 90 | 267 | 783 |
Standard deviation | 6.6 | 15.17 | 38.06 | 7.92 | 19.49 | 36.8 |
CV(%) | 7.5% | 5.4% | 4.4% | 8.8% | 7.3% | 4.7% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.088 | 0.134 | 0.176 | 0.265 | 0.402 | 0.702 | 1.263 | 2.099 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)