Human IL-1 alpha/IL1A ELISA Kit

96T/2280

Hematopoietin 1; IL 1 alpha; IL 1A; IL1; IL-1; IL1 ALPHA; IL-1 alpha; IL1A; IL1F1; IL-1α; Interleukin 1 alpha; interleukin 1, alpha; IL-1A

E.coli,S113-A271

There is no detectable cross-reactivity with other relevant proteins.

4.7 pg/ml - 300 pg/ml

IL1A

interleukin 1, alpha

P01583  

Human IL-1 alpha/IL1A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0389)

Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.

20-25分钟

Catalog number	Description	Quantity
EK0389-CAP	96-well plate precoated with anti- human IL-1 alpha antibody	1
EK0389-ST	Lyophilized recombinant human IL-1 alpha standard	10ng/tubex2
EK0389-DA	Biotinylated anti- human IL-1 alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	14	52	99	15	49	103
Standard deviation	0.98	2.49	4.35	1.06	2.4	6.07
CV(%)	7	4.8	4.4	7.1	4.9	5.9

Concentration(pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.035	0.084	0.137	0.227	0.403	0.753	1.257	2.122

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h