Rat IL-1 alpha/IL1A ELISA Kit

96T/2280

Hematopoietin 1; IL 1 alpha; IL 1A; IL1; IL-1; IL1 ALPHA; IL-1 alpha; IL1A; IL1F1; IL-1α; Interleukin 1 alpha; interleukin 1, alpha; IL-1A

Expression system for standard: E.coli; Immunogen sequence: S115-S270

There is no detectable cross-reactivity with other relevant proteins.

4.69pg/ml-300pg/ml

IL1A

interleukin 1, alpha

P16598  

Rat IL-1 alpha/IL1A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0390)

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa.

Catalog number	Description	Quantity
EK0390-CAP	96-well plate precoated with anti- rat IL-1 alpha antibody	1
EK0390-ST	Lyophilized recombinant rat IL-1 alpha standard	10ng/tubex2
EK0390-DA	Biotinylated anti- rat IL-1 alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	15	40	131	14	43	120
Standard deviation	1.06	2.12	9.69	1.09	2.58	10.8
CV(%)	7.1%	5.3%	7.4%	7.8%	6%	9%

Concentration(pg/ml)	0	4.69	9.4	18.8	37.5	75	150	300
O.D.	0.040	0.110	0.157	0.306	0.588	1.013	1.552	2.148

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum