指标别名
B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6
免疫原
Expression system for standard: E.coli; Immunogen sequence: F25-T211
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL6 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0412)
背景资料
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
试剂盒内容
Catalog number | Description | Quantity |
EK0412-CAP | Anti-Rat Il6 Pre-coated 96-well strip microplate | 1 |
EK0412-ST | Rat Il6 Standard | 10ng/tube×2 |
EK0412-DA | Rat Il6 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 193 | 579 | 1531 | 210 | 564 | 1466 |
Standard deviation | 9.65 | 35.89 | 90.32 | 11.55 | 40.04 | 109.95 |
CV(%) | 5% | 6.2% | 5.9% | 5.5% | 7.1% | 7.5% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.036 | 0.082 | 0.140 | 0.198 | 0.373 | 0.751 | 1.292 | 2.032 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)