Mouse IL10 ELISA Kit

96T/2280

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Expression system for standard: E.coli; Immunogen sequence: S19-S178

There is no detectable cross-reactivity with other relevant proteins.

15.6pg/ml-1000pg/ml

IL10

Interleukin-10

P18893  

Mouse IL10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0417)

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.

Catalog number	Description	Quantity
EK0417-CAP	Anti-Mouse Il10 Pre-coated 96-well strip microplate	1
EK0417-ST	Mouse Il10 Standard	10ng/tube×2
EK0417-DA	Mouse Il10 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	219	466	35	203	420
Standard deviation	1.88	14.01	19.1	2.66	16.64	25.7
CV(%)	5.7%	6.4%	4.1%	7.6%	8.2%	6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.051	0.095	0.165	0.267	0.450	0.758	1.278	1.840

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h