Rat IL10 ELISA Kit

96T/2280

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Expression system for standard: E.coli; Immunogen sequence: S19-N178

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

IL10

interleukin 10

P29456  

Rat IL10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0418)

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

20-25分钟

Catalog number	Description	Quantity
EK0418-CAP	96-well plate precoated with anti- rat IL-10 antibody	1
EK0418-ST	Lyophilized recombinant rat IL-10 standard	10ng/tubex2
EK0418-DA	Biotinylated anti- rat IL-10 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	55	213	839	51	222	910
Standard deviation	4.34	9.58	36.07	4.74	12.21	39.13
CV(%)	7.9%	4.5%	4.3%	9.3%	5.5%	4.3%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.071	0.102	0.137	0.228	0.413	0.687	1.261	2.064

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (EDTA)