产品名称
Human IL-17/IL17A ELISA Kit
指标别名
CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human IL-17/IL17A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0430)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.
试剂盒内容
Catalog number | Description | Quantity |
EK0430-CAP | 96-well plate precoated with anti- human IL-17 antibody | 1 |
EK0430-ST | Lyophilized recombinant human IL-17 standard | 10ng/tubex2 |
EK0430-DA | Biotinylated anti- human IL-17 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 54 | 276 | 763 | 52 | 257 | 703 |
Standard deviation | 3.88 | 19.59 | 34.33 | 4.52 | 18.5 | 37.96 |
CV(%) | 7.2% | 7.1% | 4.5% | 8.7% | 7.2% | 5.4% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.034 | 0.106 | 0.177 | 0.293 | 0.555 | 0.978 | 1.511 | 2.113 |
样本类型
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).