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Human IL-17/IL17A ELISA Kit

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EK0430

  • 96T ¥2280
  • 货期: 现货
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产品名称
Human IL-17/IL17A ELISA Kit
规格/价格
96T/2280
指标别名
CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A
免疫原
E.coli, I20-A155
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
IL17A
基因名全称
interleukin 17A
Uniprot ID
Q16552  
文献引用格式
Human IL-17/IL17A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0430)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0430-CAP96-well plate precoated with anti- human IL-17 antibody1
EK0430-STLyophilized recombinant human IL-17 standard10ng/tubex2
EK0430-DABiotinylated anti- human IL-17 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)5427676352257703
Standard deviation3.8819.5934.334.5218.537.96
CV(%)7.2%7.1%4.5%8.7%7.2%5.4%
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0340.1060.1770.2930.5550.9781.5112.113
检测方法
酶联免疫双抗夹心法
样本类型
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

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