产品名称
Mouse IL-17/IL17A EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A
免疫原
Expression system for standard: E.coli; Immunogen sequence: T22-A158
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1,000 pg/ml
文献引用格式
Mouse IL-17/IL17A EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0431)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
试剂盒内容
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
| EZ0431-CA | Mouse anti- mouse Il17a monoclonal antibody (Capture Antibody) | 500 µl, 4 µg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ0431-DA | Biotinylated goat anti- mouse Il17a polyclonal antibody (Detection Antibody) | 500 µl, 0.15 µg/mL (recommended dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) |
| EK0431-ST | Lyophilized recombinant mouse Il17a standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within theexpiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.054 | 0.1 | 0.145 | 0.225 | 0.405 | 0.755 | 1.338 | 2.185 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).
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