Mouse MDC/CCL22 ELISA Kit

96T/2480

A-152E5.1; ABCD-1; DC/B-CK; MDC; SCYA22; STCP-1; CCL22

Expression system for standard: E.coli; Immunogen sequence: G25-S92

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

CCL22

C-C motif chemokine 22

O88430  

Mouse MDC/CCL22 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0447)

Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Catalog number	Description	Quantity
EK0447-CAP	96-well plate precoated with anti- mouse MDC antibody	1
EK0447-ST	Lyophilized recombinant mouse MDC standard	10ng/tubex2
EK0447-DA	Biotinylated anti- mouse MDC antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	46	233	820	52	308	871
Standard deviation	2.94	17.48	50.84	4.11	25.26	66.20
CV(%)	6.4%	7.5%	6.2%	7.9%	8.2%	7.6%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.016	0.035	0.046	0.086	0.186	0.500	1.146	2.111

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h