产品名称
Human MIP-1 beta/CCL4 ELISA Kit
指标别名
ACT 2; ACT2; CCL4; MIP 1 beta; HC21; LAG-1; MIP1B; SCYA4; G-26; LAG1; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
基因名全称
chemokine (C-C motif) ligand 4
文献引用格式
Human MIP-1 beta/CCL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0450)
背景资料
Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0450-CAP | 96-well plate precoated with anti- human CCL4 antibody | 1 |
EK0450-ST | Lyophilized recombinant human CCL4 standard | 10ng/tubex2 |
EK0450-DA | Biotinylated anti- human CCL4 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 13 | 90 | 254 | 12 | 95 | 256 |
Standard deviation | 0.63 | 4.95 | 13.2 | 0.62 | 6.84 | 15.87 |
CV(%) | 4.9 | 5.5 | 5.2 | 5.2 | 7.2 | 6.2 |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.016 | 0.058 | 0.110 | 0.197 | 0.423 | 0.756 | 1.247 | 1.807 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)