产品名称
Rat MIP-1 beta/CCL4 ELISA Kit
指标别名
ACT 2; ACT2; CCL4; MIP 1 beta; HC21; LAG-1; MIP1B; SCYA4; G-26; LAG1; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-N92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 4
文献引用格式
Rat MIP-1 beta/CCL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0939)
背景资料
Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).
试剂盒内容
Catalog number | Description | Quantity |
EK0939-CAP | 96-well plate precoated with anti- rat CCL4 antibody | 1 |
EK0939-ST | Lyophilized recombinant rat CCL4 standard | 10ng/tube×2 |
EK0939-DA | Biotinylated anti- rat CCL4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 144 | 453 | 31 | 148 | 419 |
Standard deviation | 1.42 | 9.21 | 24.91 | 1.67 | 12.13 | 24.3 |
CV(%) | 4.6% | 6.4% | 5.5% | 5.4% | 8.2% | 5.8% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.005 | 0.112 | 0.234 | 0.450 | 0.835 | 1.179 | 1.667 | 2.174 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)