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Rat MIP-1 beta/CCL4 ELISA Kit

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EK0939

  • 96T ¥2480
  • 货期: 现货

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产品名称
Rat MIP-1 beta/CCL4 ELISA Kit
规格/价格
96T/2480
指标别名
ACT 2; ACT2; CCL4; MIP 1 beta; HC21; LAG-1; MIP1B; SCYA4; G-26; LAG1; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-N92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6pg/ml-1000pg/ml
基因名称
CCL4
基因名全称
chemokine (C-C motif) ligand 4
Uniprot ID
P50230  
文献引用格式
Rat MIP-1 beta/CCL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0939)
背景资料
Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0939-CAP96-well plate precoated with anti- rat CCL4 antibody1
EK0939-STLyophilized recombinant rat CCL4 standard10ng/tube×2
EK0939-DABiotinylated anti- rat CCL4 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)3114445331148419
Standard deviation1.429.2124.911.6712.1324.3
CV(%)4.6%6.4%5.5%5.4%8.2%5.8%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0050.1120.2340.4500.8351.1791.6672.174
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

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