Human MIP-3 alpha/CCL20 ELISA Kit

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EK0453

96T ￥2480
货期：现货

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Human MIP-3 alpha/CCL20 ELISA Kit standard curve
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产品简介>

产品名称

Human MIP-3 alpha/CCL20 ELISA Kit

规格/价格

96T|2480

指标别名

Beta chemokine exodus 1; C C motif chemokine 20; CC chemokine LARC; CCL20; CKb4; LARC; MIP 3 alpha; MIP 3a; MIP3A; MIP-3α; SCYA20; Small inducible cytokine A20; ST38

免疫原

E.coli, A27-M96

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

7.8 pg/ml - 500 pg/ml

基因名称

CCL20

基因名全称

chemokine (C-C motif) ligand 20

Uniprot ID

P78556

文献引用格式

Human MIP-3 alpha/CCL20 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0453)

背景资料

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0453-CAP	96-well plate precoated with anti- human MIP-3 alpha antibody	1
EK0453-ST	Lyophilized recombinant human MIP-3 alpha standard	10ng/tubex2
EK0453-DA	Biotinylated anti- human MIP-3 alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	19	78	256	17	73	269
Standard deviation	1.34	4.91	13.05	1.22	5.03	15.06
CV(%)	7.1	6.3	5.1	7.2	6.9	5.6

典型数据示例

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.030	0.106	0.205	0.339	0.581	0.952	1.520	2.198

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human MIP-3 alpha/CCL20 ELISA Kit

EK0453

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(10) All Species Human(10) 清除

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Human MIP-3 alpha/CCL20 ELISA Kit

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