产品名称
Mouse MIP-3 alpha/CCL20 ELISA Kit
指标别名
Beta chemokine exodus 1; C C motif chemokine 20; CC chemokine LARC; CCL20; CKb4; LARC; MIP 3 alpha; MIP 3a; MIP3A; MIP-3α; SCYA20; Small inducible cytokine A20; ST38
免疫原
Expression system for standard: E.coli; Immunogen sequence: A28-M97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 20
文献引用格式
Mouse MIP-3 alpha/CCL20 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0454)
背景资料
Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0454-CAP | 96-well plate precoated with anti- mouse CCL20 antibody | 1 |
EK0454-ST | Lyophilized recombinant mouse CCL20 standard | 10ng/tubex2 |
EK0454-DA | Biotinylated anti- mouse CCL20 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 9 | 55 | 313 | 9 | 57 | 308 |
Standard deviation | 0.62 | 3.02 | 21.28 | 0.78 | 3.7 | 21.56 |
CV(%) | 6.9% | 5.5% | 6.8% | 8.7% | 6.5% | 7% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.001 | 0.090 | 0.183 | 0.357 | 0.644 | 1.136 | 1.780 | 2.179 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)