Human NGF beta/NGF ELISA Kit

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EK0469

96T ￥2280
货期：现货

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产品名称

Human NGF beta/NGF ELISA Kit

规格/价格

96T/2280

指标别名

NGF; NGFB; Beta-nerve growth factor; Beta-NGF

免疫原

NSO, S122-A241

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

NGF

基因名全称

nerve growth factor (beta polypeptide)

Uniprot ID

P01138

文献引用格式

Human NGF beta/NGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0469)

背景资料

Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0469-CAP	96-well plate precoated with anti- human NGF antibody	1
EK0469-ST	Lyophilized recombinant human NGF standard	10ng/tubex2
EK0469-DA	Biotinylated anti- human NGF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	216	468	30	203	429
Standard deviation	1.38	14.47	20.59	1.8	13.8	22.3
CV(%)	4.2	6.7	4.4	6	6.8	5.2

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.062	0.091	0.133	0.225	0.403	0.735	1.165	2.059

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human NGF beta/NGF ELISA Kit

EK0469

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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