Rat NGF beta/NGF ELISA Kit

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EK0471

96T ￥2280
货期：现货

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Rat NGF/NGF beta ELISA Kit standard curve
all(2)
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产品简介>

产品名称

Rat NGF beta/NGF ELISA Kit

规格/价格

96T/2280

指标别名

nerve growth factor; Beta-NGF; HSAN5B; NGF; NGF beta

免疫原

Expression system for standard: NS0; Immunogen sequence: S122-G241

交叉反应

There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

检测范围

15.6pg/ml-1000pg/ml

基因名称

NGF

基因名全称

nerve growth factor (beta polypeptide)

Uniprot ID

P25427

文献引用格式

Rat NGF beta/NGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0471)

背景资料

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0471-CAP	96-well plate precoated with anti- rat NGF antibody	1
EK0471-ST	Lyophilized recombinant rat NGF standard	10ng/tubex2
EK0471-DA	Biotinylated anti- rat NGF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	191	480	29	201	491
Standard deviation	1.84	8.02	32.64	1.62	11.65	35.84
CV(%)	5.6%	4.2%	6.8%	5.6%	5.8%	7.3%

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.018	0.074	0.141	0.269	0.544	0.977	1.605	2.343

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat NGF beta/NGF ELISA Kit

EK0471

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

其他介绍>

引用文献(0)>

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EK0469

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Rat NGF beta/NGF ELISA Kit

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