Human Osteopontin/SPP1 ELISA Kit

96T/2480

BNSP; Bone sialoprotein 1; BSPI; ETA 1; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1; SPP 1; SPP1; Urinary stone protein; Uropontin

NSO, I17-N300

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10000 pg/ml

SPP1

secreted phosphoprotein 1

P10451  

Human Osteopontin/SPP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0482)

Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

15-20分钟

Catalog number	Description	Quantity
EK0482-CAP	96-well plate precoated with anti- human OPN antibody	1
EK0482-ST	Lyophilized recombinant human OPN standard	10ng/tubex2
EK0482-DA	Biotinylated anti- human OPN antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	305	1771	4985	314	1611	5408
Standard deviation	14.03	138.13	304.08	16.64	133.71	383.96
CV(%)	4.6	7.8	6.1	5.3	8.3	7.1

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.138	0.173	0.195	0.224	0.415	1.011	1.585	2.299

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Human Milk and Urine

3.5h