Mouse Osteopontin/SPP1 ELISA Kit

96T/2480

BNSP; Bone sialoprotein 1; BSPI; ETA 1; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1; SPP 1; SPP1; Urinary stone protein; Uropontin

Expression system for standard: NS0; Immunogen sequence: L17-N294

There is no detectable cross-reactivity with other relevant proteins.

156pg/ml-10000pg/ml

SPP1

secreted phosphoprotein 1

Q3UBR1  

Mouse Osteopontin/SPP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0483)

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.

Catalog number	Description	Quantity
EK0483-CAP	96-well plate precoated with anti- mouse OPN antibody	1
EK0483-ST	Lyophilized recombinant mouse OPN standard	10ng/tubex2
EK0483-DA	Biotinylated anti- mouse OPN antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	306	1986	5401	308	2000	4959
Standard deviation	14.68	117.17	405.07	15.09	142	446.31
CV(%)	4.8%	5.9%	7.5%	4.9%	7.1%	9%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.046	0.116	0.198	0.305	0.483	0.818	1.503	2.032

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine

3.5h