产品名称
Human PLGF/PGF ELISA Kit
指标别名
D12S1900; PGF; PGFL; PIGF; Placenta growth factor; placental growth factor; PLGF; PlGF 2; SHGC 10760
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
placental growth factor
文献引用格式
Human PLGF/PGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0490)
背景资料
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0490-CAP | 96-well plate precoated with anti- human PLGF antibody | 1 |
EK0490-ST | Lyophilized recombinant human PLGF standard | 10ng/tubex2 |
EK0490-DA | Biotinylated anti- human PLGF antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 221 | 454 | 37 | 215 | 459 |
Standard deviation | 3.16 | 13.26 | 23.15 | 3.51 | 13.76 | 25.7 |
CV(%) | 7.9 | 6 | 5.1 | 9.5 | 6.4 | 5.6 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.052 | 0.105 | 0.155 | 0.249 | 0.412 | 0.819 | 1.380 | 2.112 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine