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Human PLGF/PGF ELISA Kit

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EK0490

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human PLGF/PGF ELISA Kit
规格/价格
96T/2480
指标别名
D12S1900; PGF; PGFL; PIGF; Placenta growth factor; placental growth factor; PLGF; PlGF 2; SHGC 10760
免疫原
E.coli, A21-R149
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
PGF
基因名全称
placental growth factor
Uniprot ID
P49763  
文献引用格式
Human PLGF/PGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0490)
背景资料
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0490-CAP96-well plate precoated with anti- human PLGF antibody1
EK0490-STLyophilized recombinant human PLGF standard10ng/tubex2
EK0490-DABiotinylated anti- human PLGF antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4022145437215459
Standard deviation3.1613.2623.153.5113.7625.7
CV(%)7.965.19.56.45.6
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0520.1050.1550.2490.4120.8191.3802.112
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine
实验时长
3.5h

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