产品名称
Mouse PLGF-2/PGF ELISA Kit
指标别名
D12S1900; PGF; PGFL; PIGF; Placenta growth factor; placental growth factor; PLGF; PlGF 2; SHGC 10760
免疫原
Expression system for standard: NS0; Immunogen sequence: A24-P158
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
placental growth factor
文献引用格式
Mouse PLGF-2/PGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0863)
背景资料
Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0863-CAP | 96-well plate precoated with anti- mouse PLGF-2 antibody | 1 |
EK0863-ST | Lyophilized recombinant mouse PLGF-2 standard | 10ng/tube×2 |
EK0863-DA | Biotinylated anti- mouse PLGF-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 176 | 474 | 34 | 182 | 481 |
Standard deviation | 2.01 | 12.14 | 20.38 | 2.24 | 13.83 | 20.68 |
CV(%) | 6.1% | 6.9% | 4.3% | 6.6% | 7.6% | 4.3% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.054 | 0.106 | 0.162 | 0.284 | 0.563 | 0.896 | 1.618 | 2.259 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)