产品名称
Human SDF-1/CXCL12 ELISA Kit
指标别名
C X C motif chemokine 12; CXCL12; CXCL12/SDF-1; hIRH; hSDF 1; IRH; PBSF; SCYB12; SDF 1; SDF 1a; SDF 1b; SDF1; SDF-1; SDF1A; SDF1B; Stromal cell derived factor 1; TLSF a; TLSF b; TPAR1
免疫原
Expression system for standard: E.coli; Immunogen sequence: V24-M93
基因名全称
C-X-C motif chemokine ligand 12
文献引用格式
Human SDF-1/CXCL12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0499)
背景资料
The stromal cell-derived factor 1 (SDF-1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that in humans is encoded by the CXCL12 gene on chromosome 10. This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0499-CAP | 96-well plate precoated with anti- Human SDF-1/CXCL12 ELISA Kit A antibody | 1 |
EK0499-ST | Lyophilized recombinant Human SDF-1/CXCL12 ELISA Kit A standard | 1ng/tube×2 |
EK0499 -DA | Biotinylated anti- Human SDF-1/CXCL12 ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 112 | 498 | 1863 | 122 | 479 | 1863 |
Standard deviation | 4.93 | 20.42 | 96.88 | 3.9 | 22.03 | 72.66 |
CV(%) | 4.4 | 4.1 | 5.2 | 3.2 | 4.6 | 3.9 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.022 | 0.057 | 0.173 | 0.395 | 0.854 | 1.291 | 1.825 | 2.342 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
蛋白名全称
Stromal cell-derived factor 1
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)