Mouse SDF-1/CXCL12 ELISA Kit

96T/2480

C X C motif chemokine 12; CXCL12; CXCL12/SDF-1; hIRH; hSDF 1; IRH; PBSF; SCYB12; SDF 1; SDF 1a; SDF 1b; SDF1; SDF-1; SDF1A; SDF1B; Stromal cell derived factor 1; TLSF a; TLSF b; TPAR1

Expression system for standard: E.coli; Immunogen sequence: K22-M93

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

CXCL12

chemokine (C-X-C motif) ligand 12

H7BX38  

Mouse SDF-1/CXCL12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0500)

SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.

15-20分钟

Catalog number	Description	Quantity
EK0500-CAP	96-well plate precoated with anti- Mouse SDF-1/CXCL12 ELISA Kit A antibody	1
EK0500-ST	Lyophilized recombinant Mouse SDF-1/CXCL12 ELISA Kit A standard	1ng/tube×2
EK0500 -DA	Biotinylated anti- Mouse SDF-1/CXCL12 ELISA Kit A antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	118	692	1469	115	707	1562
Standard deviation	5.31	49.13	60.23	5.64	36.76	90.6
CV (%)	4.5%	7.1%	4.1%	4.9%	5.2%	5.8%

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.039	0.072	0.119	0.198	0.431	0.675	1.352	2.023

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin)

3.5h