产品名称
Human TIMP4 ELISA Kit
指标别名
Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP-4
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名全称
TIMP metallopeptidase inhibitor 4
文献引用格式
Human TIMP4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0524)
背景资料
Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0524-CAP | 96-well plate precoated with anti- human TIMP-4 antibody | 1 |
EK0524-ST | Lyophilized recombinant human TIMP-4 standard | 10ng/tubex2 |
EK0524-DA | Biotinylated anti- human TIMP-4 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 123 | 589 | 2056 | 122 | 601 | 1963 |
Standard deviation | 9.47 | 38.87 | 125.41 | 10.98 | 48.08 | 155.07 |
CV(%) | 7.7 | 6.6 | 6.1 | 9 | 8 | 7.9 |
典型数据示例
Concentration(pg/ml) | 0.0 78 | 156 | 313 | 625 | 1250 | 2500 | 5,000 |
O.D. | 0.054 | 0.140 | 0.206 | 0.375 | 0.660 | 1.127 | 1.843 | 2.229 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Human Milk