产品名称
Mouse TIMP4 ELISA Kit
指标别名
Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP-4
免疫原
Expression system for standard: NS0; Immunogen sequence: C30-P224
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Metalloproteinase inhibitor 4
文献引用格式
Mouse TIMP4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1335)
背景资料
Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1335-CAP | 96-well plate precoated with anti- mouse TIMP-4 antibody | 1 |
EK1335-ST | Lyophilized recombinant mouse TIMP-4 standard | 10ng/tube×2 |
EK1335-DA | Biotinylated anti- mouse TIMP-4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 131 | 690 | 2000 | 137 | 710 | 2055 |
Standard deviation | 6.94 | 44.85 | 156 | 8.63 | 51.83 | 199.33 |
CV(%) | 5.3% | 6.5% | 7.8% | 6.3% | 7.3% | 9.7% |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.064 | 0.169 | 0.232 | 0.339 | 0.567 | 0.965 | 1.465 | 1.947 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)