Human VCAM1 ELISA Kit

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EK0537

96T ￥2280
货期：现货

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Human VCAM-1 ELISA Kit standard curve
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产品简介>

产品名称

Human VCAM1 ELISA Kit

规格/价格

96T/2280

指标别名

CD106; INCAM 100; V CAM 1; VCAM 1; VCAM1

免疫原

NSO,F25 - E698

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

VCAM1

基因名全称

vascular cell adhesion molecule 1

Uniprot ID

P19320

文献引用格式

Human VCAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0537)

背景资料

Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0537-CAP	96-well plate precoated with anti- human VCAM-1 antibody	1
EK0537-ST	Lyophilized recombinant human VCAM-1 standard	10ng/tubex2
EK0537-DA	Biotinylated anti- human VCAM-1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	310	1065	5287	304	1029	5603
Standard deviation	17.67	51.12	385.95	19.15	65.85	481.85
CV(%)	5.7	4.8	7.3	6.3	6.4	8.6

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.067	0.129	0.224	0.419	0.642	1.179	1.807	2.284

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human VCAM1 ELISA Kit

EK0537

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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EK0538

EZ0537

EZ0538

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Human VCAM1 ELISA Kit

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