Mouse VCAM1 ELISA Kit

96T/2280

CD106; INCAM 100; V CAM 1; VCAM 1; VCAM1

Expression system for standard: NS0; Immunogen sequence: F25-E698

There is no detectable cross-reactivity with other relevant proteins.

156pg/ml-10000pg/ml

VCAM1

Vascular cell adhesion protein 1

P29533  

Mouse VCAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0538)

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.

Catalog number	Description	Quantity
EK0538-CAP	96-well plate precoated with anti- mouse VCAM-1 antibody	1
EK0538-ST	Lyophilized recombinant mouse VCAM-1 standard	10ng/tubex2
EK0538-DA	Biotinylated anti- mouse VCAM-1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	371	1198	4387	354	1298	4119
Standard deviation	25.22	86.25	298.31	29.02	112.92	317.16
CV(%)	6.8%	7.2%	6.8%	8.2%	8.7%	7.7%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.013	0.102	0.180	0.337	0.631	1.156	1.728	2.457

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h