Rat VEGF/VEGFA ELISA Kit

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EK0540

96T ￥2280
货期：现货

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Rat VEGF ELISA Kit standard curve
all(2)
all(2)

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产品简介>

产品名称

Rat VEGF/VEGFA ELISA Kit

规格/价格

96T/2280

指标别名

L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF

免疫原

Expression system for standard: NS0; Immunogen sequence: A27-R190

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

15.6pg/ml-1000pg/ml

基因名称

VEGFA

基因名全称

vascular endothelial growth factor A

Uniprot ID

P16612

文献引用格式

Rat VEGF/VEGFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0540)

背景资料

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0540-CAP	96-well plate precoated with anti- rat VEGF antibody	1
EK0540-ST	Lyophilized recombinant rat VEGF standard	10ng/tubex2
EK0540-DA	Biotinylated anti- rat VEGF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	29	243	389	31	234	368
Standard deviation	1.91	18.95	16.72	2.07	18.25	17.66
CV(%)	6.6%	7.8%	4.3%	6.7%	7.8%	4.8%

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.023	0.075	0.119	0.234	0.401	0.804	1.433	1.971

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat VEGF/VEGFA ELISA Kit

EK0540

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

其他介绍>

引用文献(0)>

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Rat VEGF/VEGFA ELISA Kit

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