产品名称
Mouse VEGF/VEGFA Quick ELISA Kit
指标别名
L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF
免疫原
Expression system for standard: NS0; Immunogen sequence: A27-R190
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
vascular endothelial growth factor A
文献引用格式
Mouse VEGF/VEGFA Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0541)
背景资料
Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
试剂盒内容
Catalog number | Description | Quantity |
FEK0541-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0541-ST | Mouse VEGF standard | 10ng/tube×2 |
FEK0541-A | Mouse VEGF Antibody A | 3ml |
FEK0541-B | Mouse VEGF Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
CV(%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.096 | 0.178 | 0.358 | 0.595 | 0.854 | 1.369 | 1.877 | 2.329 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)