产品名称
Human VEGFR2/KDR ELISA Kit
指标别名
CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
kinase insert domain receptor (a type III receptor tyrosine kinase)
文献引用格式
Human VEGFR2/KDR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0544)
背景资料
Vascular endothelial growth factor receptor 2(VEGFR-2) also known as Kinase insert domain receptor(KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0544-CAP | 96-well plate precoated with anti- human VEGFR2 antibody | 1 |
EK0544-ST | Lyophilized recombinant human VEGFR2 standard | 10ng/tubex2 |
EK0544-DA | Biotinylated anti- human VEGFR2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 177 | 400 | 1762 | 167 | 410 | 1810 |
Standard deviation | 10.79 | 26 | 137.43 | 11.52 | 29.52 | 161.09 |
CV(%) | 6.1 | 6.5 | 7.8 | 6.9 | 7.2 | 8.9 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.065 | 0.115 | 0.165 | 0.265 | 0.446 | 0.814 | 1.517 | 2.462 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)