产品名称
Mouse VEGFR2/KDR ELISA Kit
指标别名
CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2
免疫原
Expression system for standard: NS0; Immunogen sequence: A20-E762
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Vascular endothelial growth factor receptor 2
文献引用格式
Mouse VEGFR2/KDR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0590)
背景资料
Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0590-CAP | 96-well plate precoated with anti- mouse VEGFR2 antibody | 1 |
EK0590-ST | Lyophilized recombinant mouse VEGFR2 standard | 10ng/tubex2 |
EK0590-DA | Biotinylated anti- mouse VEGFR2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 134 | 585 | 1309 | 133 | 543 | 1323 |
Standard deviation | 6.83 | 36.27 | 60.21 | 9.31 | 39.09 | 63.5 |
CV(%) | 5.1% | 6.2% | 4.6% | 7% | 7.2% | 4.8% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.010 | 0.031 | 0.059 | 0.119 | 0.287 | 0.720 | 1.590 | 2.334 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum