产品名称
Human CCL21 ELISA Kit
指标别名
C-C motif chemokine ligand 21; 6Ckine; CKb9; ECL; SCYA21; SLC; TCA4; CCL21
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
chemokine (C-C motif) ligand 21
文献引用格式
Human CCL21 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0555)
背景资料
Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0555-CAP | 96-well plate precoated with anti- human CCL21 antibody | 1 |
EK0555-ST | Lyophilized recombinant human CCL21 standard | 10ng/tubex2 |
EK0555-DA | Biotinylated anti- human CCL21 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 100 | 709 | 1352 | 103 | 706 | 1475 |
Standard deviation | 5.6 | 28.36 | 82.47 | 6.9 | 31.77 | 92.92 |
CV(%) | 5.6 | 4 | 6.1 | 6.7 | 4.5 | 6.3 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.031 | 0.057 | 0.102 | 0.203 | 0.408 | 0.504 | 1.178 | 2.318 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)