产品名称
Human CCL21 Quick ELISA Kit
指标别名
C-C motif chemokine ligand 21; 6Ckine; CKb9; ECL; SCYA21; SLC; TCA4; CCL21
免疫原
Expression system for standard: E.coli; Immunogen sequence: S24-P134
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 21
文献引用格式
Human CCL21 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0555)
背景资料
Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Human CCL21 antibody | 1 |
FEK0663-ST | lyophilized recombinant Human CCL21 standard | 10ng/tube×2 |
FEK0663-A | Human CCL21 Antibody A | 3ml |
FEK0555-B | Human CCL21 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 117 | 825 | 1412 | 108 | 794 | 1510 |
Standard deviation | 4.56 | 30.53 | 83.31 | 7.02 | 34.94 | 92.11 |
CV (%) | 3.9% | 3.7% | 5.9% | 6.5% | 4.4% | 6.1% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.010 | 0.139 | 0.313 | 0.475 | 0.802 | 1.584 | 2.396 | 2.608 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)