Human E-cadherin/CDH1 ELISA Kit

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EK0561

96T ￥2480
货期：现货

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Human E-Cadherin ELISA Kit standard curve
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产品简介>

产品名称

Human E-cadherin/CDH1 ELISA Kit

规格/价格

96T/2480

指标别名

Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin

免疫原

NSO, D155-I707

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

CDH1

基因名全称

cadherin 1, type 1, E-cadherin (epithelial)

Uniprot ID

P12830

文献引用格式

Human E-cadherin/CDH1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0561)

背景资料

E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0561-CAP	96-well plate precoated with anti- human E-Cadherin antibody	1
EK0561-ST	Lyophilized recombinant human E-Cadherin standard	10ng/tubex2
EK0561-DA	Biotinylated anti- human E-Cadherin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	265	1264	4789	253	1258	4691
Standard deviation	15.37	58.14	325.65	16.69	61.64	318.98
CV(%)	5.8	4.6	6.8	6.6	4.9	6.8

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.021	0.079	0.142	0.266	0.483	0.838	1.510	2.124

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human E-cadherin/CDH1 ELISA Kit

EK0561

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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EK0562

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Human E-cadherin/CDH1 ELISA Kit

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