产品名称
Mouse E-cadherin/CDH1 ELISA Kit
指标别名
Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin
免疫原
Expression system for standard: NS0; Immunogen sequence: D157-V709
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse E-cadherin/CDH1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0562)
背景资料
E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0562-CAP | 96-well plate precoated with anti- mouse E-Cadherin antibody | 1 |
EK0562-ST | Lyophilized recombinant mouse E-Cadherin standard | 10ng/tubex2 |
EK0562-DA | Biotinylated anti- mouse E-Cadherin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 242 | 1644 | 5631 | 217 | 1771 | 5410 |
Standard deviation | 14.52 | 118.36 | 360.38 | 14.53 | 146.99 | 394.93 |
CV(%) | 6% | 7.2% | 6.4% | 6.7% | 8.3% | 7.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.015 | 0.076 | 0.134 | 0.239 | 0.509 | 0.904 | 1.457 | 2.265 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)