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Human LIF ELISA Kit

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EK0579

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human LIF ELISA Kit
规格/价格
96T/2480
指标别名
CDF; D factor; DIA; Emfilermin; HILDA; Leukemia inhibitory factor; LIF; Melanoma derived LPL inhibitor; MLPLI
免疫原
Expression system for standard: E.coli; Immunogen sequence: S23-F202
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1,000 pg/ml
基因名称
LIF
基因名全称
LIF interleukin 6 family cytokine
Uniprot ID
P15018  
文献引用格式
Human LIF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0579)
背景资料
Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0579-CAP96-well plate precoated with anti- Human LIF ELISA Kit A antibody1
EK0579-STLyophilized recombinant Human LIF ELISA Kit A standard10ng/tube×2
EK0579 -DABiotinylated anti- Human LIF ELISA Kit A antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)23403413054431
Standard deviation0.62.1418.960.791.7318.02
CV(%)2.6%5.3%5.6%2.6%3.2%4.2%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001,000
O.D.0.0150.0380.1550.2610.4240.8161.2342.028
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
Gene ID
3976  
样本类型
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
实验时长
3.5h

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