指标别名
CDF; D factor; DIA; Emfilermin; HILDA; Leukemia inhibitory factor; LIF; Melanoma derived LPL inhibitor; MLPLI
免疫原
Expression system for standard: E.coli; Immunogen sequence: S23-F202
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1,000 pg/ml
基因名全称
LIF interleukin 6 family cytokine
文献引用格式
Human LIF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0579)
背景资料
Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
试剂盒内容
Catalog number | Description | Quantity |
EK0579-CAP | 96-well plate precoated with anti- Human LIF ELISA Kit A antibody | 1 |
EK0579-ST | Lyophilized recombinant Human LIF ELISA Kit A standard | 10ng/tube×2 |
EK0579 -DA | Biotinylated anti- Human LIF ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 23 | 40 | 341 | 30 | 54 | 431 |
Standard deviation | 0.6 | 2.14 | 18.96 | 0.79 | 1.73 | 18.02 |
CV(%) | 2.6% | 5.3% | 5.6% | 2.6% | 3.2% | 4.2% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.015 | 0.038 | 0.155 | 0.261 | 0.424 | 0.816 | 1.234 | 2.028 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.