Mouse LIF ELISA Kit

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EK0580

96T ￥2480
货期：现货

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Mouse LIF ELISA Kit standard curve
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产品简介>

产品名称

Mouse LIF ELISA Kit

规格/价格

96T/2480

指标别名

CDF; D factor; DIA; Emfilermin; HILDA; Leukemia inhibitory factor; LIF; Melanoma derived LPL inhibitor; MLPLI

免疫原

Expression system for standard: E.coli; Immunogen sequence: S24-F203

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

7.8pg/ml-500pg/ml

基因名称

Lif

基因名全称

Leukemia inhibitory factor

Uniprot ID

P09056

文献引用格式

Mouse LIF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0580)

背景资料

Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.

试剂盒内容

Catalog number	Description	Quantity
EK0580-CAP	96-well plate precoated with anti- mouse LIF antibody	1
EK0580-ST	Lyophilized recombinant mouse LIF standard	1ng/tube×2
EK0580-DA	Biotinylated anti- mouse LIF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	70	272	15	76	278
Standard deviation	0.7	3.01	19.58	0.72	3.34	24.74
CV(%)	4.4%	4.3%	7.2%	4.8%	4.4%	8.9%

典型数据示例

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.008	0.062	0.141	0.295	0.574	1.100	1.981	2.761

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse LIF ELISA Kit

EK0580

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

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