指标别名
Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL18; IL-18; IL1F4; Interleukin 1 gamma; Interleukin 18; Mature IL18; pre IL18; Pre-IL18
免疫原
Expression system for standard: E.coli; Immunogen sequence: H37-S194
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL18 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0592)
背景资料
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
试剂盒内容
Catalog number | Description | Quantity |
EK0592-CAP | 96-well plate precoated with anti- rat IL-18 antibody | 1 |
EK0592-ST | Lyophilized recombinant rat IL-18 standard | 10ng/tubex2 |
EK0592-DA | Biotinylated anti- rat IL-18 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 29 | 166 | 511 | 29 | 165 | 490 |
Standard deviation | 1.97 | 7.8 | 22.99 | 2.2 | 9.07 | 29.4 |
CV(%) | 6.8% | 4.7% | 4.5% | 7.6% | 5.5% | 6% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.083 | 0.124 | 0.165 | 0.233 | 0.361 | 0.698 | 1.177 | 1.949 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)