产品名称
Human IL18 ELISA Kit
指标别名
Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL18; IL-18; IL1F4; Interleukin 1 gamma; Interleukin 18; Mature IL18; pre IL18; Pre-IL18
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human IL18 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0864)
背景资料
Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0864-CAP | 96-well plate precoated with anti- human IL-18 antibody | 1 |
EK0864-ST | Lyophilized recombinant human IL-18 standard | 10ng/tube×2 |
EK0864-DA | Biotinylated anti- human IL-18 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 247 | 520 | 36 | 254 | 548 |
Standard deviation | 2.4 | 13.83 | 21.84 | 2.66 | 16.25 | 31.78 |
CV(%) | 7.3 | 5.6 | 4.2 | 7.4 | 6.4 | 5.8 |
典型数据示例
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.033 | 0.105 | 0.132 | 0.216 | 0.364 | 0.676 | 1.235 | 1.955 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)