产品名称
Human Prolactin/PRL ELISA Kit
指标别名
prolactin; GHA1; PRL
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Prolactin/PRL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0593)
背景资料
Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0593-CAP | 96-well plate precoated with anti- human Prolactin antibody | 1 |
EK0593-ST | Lyophilized recombinant human Prolactin standard | 10ng/tubex2 |
EK0593-DA | Biotinylated anti- human Prolactin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 278 | 1989 | 4706 | 274 | 1928 | 5020 |
Standard deviation | 18.07 | 95.47 | 338.83 | 21.64 | 129.17 | 371.48 |
CV(%) | 6.5 | 4.8 | 7.2 | 7.9 | 6.7 | 7.4 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.070 | 0.143 | 0.309 | 0.518 | 1.021 | 1.595 | 1.930 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)