产品名称
Mouse Prolactin/PRL ELISA Kit
指标别名
prolactin; GHA1; PRL
免疫原
Expression system for standard: E.coli; Immunogen sequence: L32-C228
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Prolactin/PRL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0594)
背景资料
Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0594-CAP | 96-well plate precoated with anti- mouse Prolactin antibody | 1 |
EK0594-ST | Lyophilized recombinant mouse Prolactin standard | 10ng/tubex2 |
EK0594-DA | Biotinylated anti- mouse Prolactin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 247 | 1548 | 4124 | 254 | 1402 | 4314 |
Standard deviation | 13.83 | 68.11 | 206.2 | 15.74 | 82.71 | 228.64 |
CV(%) | 5.6% | 4.4% | 5% | 6.2% | 5.9% | 5.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.110 | 0.185 | 0.263 | 0.398 | 0.686 | 1.047 | 1.685 | 2.234 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, citrate)