Rat MMP2 ELISA Kit

96T/2480

72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; Gelatinase A; Matrix metalloproteinase 2; MMP 2; MMP II; MMP2; MMP-2; MONA; TBE 1

Expression system for standard: NS0; Immunogen sequence: A30-C662

There is no detectable cross-reactivity with other relevant proteins.

625pg/ml-40000pg/ml

MMP2

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

P33436  

Rat MMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0639)

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

25-30分钟

Catalog number	Description	Quantity
EK0639-CAP	96-well plate precoated with anti- rat MMP-2 antibody	1
EK0639-ST	Lyophilized recombinant rat MMP-2 standard	40ng/tubex2
EK0639-DA	Biotinylated anti- rat MMP-2 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1093	4492	23073	1098	4440	21240
Standard deviation	68.85	296.47	1107.5	90.03	368.52	1295.64
CV(%)	6.3%	6.6%	4.8%	8.2%	8.3%	6.1%

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.086	0.192	0.233	0.335	0.524	0.833	1.478	2.119

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum and Plasma (heparin)