Mouse MMP2 Quick ELISA Kit

96T/3580

72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; Gelatinase A; Matrix metalloproteinase 2; MMP 2; MMP II; MMP2; MMP-2; MONA; TBE 1

Expression system for standard: NS0; Immunogen sequence: A30-C662

There is no detectable cross-reactivity with other relevant proteins.

625pg/ml-40000pg/ml

MMP2

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

P33434  

Mouse MMP2 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0460)

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

15分钟

Catalog number	Description	Quantity
FEK0460-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK0460-ST	Mouse MMP-2 standard	10ng/tube×2
FEK0460-A	Mouse MMP-2 Antibody A	3ml
FEK0460-B	Mouse MMP-2 Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1347	3055	16379	1452	3201	16183
Standard deviation	60.61	140.53	720.67	81.31	150.44	744.41
CV(%)	4.5%	4.6%	4.4%	5.6%	4.7%	4.6%

Concentration(pg/ml)

0

625

1250

2500

5000

10000

20000

40000O.D

0.029

0.115

0.173

0.310

0.537

0.976

1.555

2.153

Store at 4℃ for 6 months.

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin)